|Reactivity||Human, Mouse, Rat|
|Calculated MW||565176 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Ryanodine receptor 1, RYR-1, RyR1, Skeletal muscle calcium release channel, Skeletal muscle ryanodine receptor, Skeletal muscle-type ryanodine receptor, Type 1 ryanodine receptor, RYR1, RYDR|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human Ryanodine Receptor 1 (Accession ֲ P21817).ֲ ֲ Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3 The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel . They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability. The RyR1 is expressed predominantly in skeletal muscles and areas of the brain; the human RyR1 has at least three known alternative spliced variants.5 Several diseases are attributed to mutations in RyR1 gene: Central Core Disease (CCD), multi-minicore disease (MmD) and Malignant Hyperthermia (MH).5
References 1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. 2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. 3. Hamilton, S.L. (2005) Cell Calcium 38, 253. 4. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893. 5. UniProtKB/Swiss-Prot entry P21817 [RYR1_HUMAN] Ryanodine receptor 1.
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