|Calculated MW||31871 Da|
|Homology||Mouse -13/16 amino acid residues identical.|
|Other Names||Aquaporin-9, AQP-9, Aquaglyceroporin-9, Aqp9|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP-9 (Accession number P56627).ֲ Intracellular, N-terminus.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12. The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include AQP-1, AQP-2, AQP4, AQP-5, AQP-6 and AQP-8, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10. The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2 AQP-9 is expressed in several tissues including brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular N-terminus domain of the rat AQP-9 channel. Anti-Aquaporin 9 antibody (#AG1041) can be used in western blot and immunohistochemical applications, and recognizes AQP-9 from rat and mouse samples.
References 1. Rojek, A.M. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3609. 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 3. Koyama, Y. et al. (1997) J Biol Chem 272, 30329.
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