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Aquaporin 9 Antibody

Affinity purified polyclonal antibody

     
  • WB - Aquaporin 9 Antibody AG1041-050
    Western blot analysis of rat brain (lanes 1and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:
    1,2,3. Anti-Aquaporin 9  antibody (#AG1041), (1:200).
    4,5,6. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
  • WB - Aquaporin 9 Antibody AG1041-050
    Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:
    1. Anti-Aquaporin 9 antibody (#AG1041), (1:200).
    2. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
  • WB - Aquaporin 9 Antibody AG1041-050
    Western blot analysis of mouse brain membranes:
    1. Anti-Aquaporin 9 antibody (#AG1041), (1:200).
    2. Anti-Aquaporin 9 antibody, preincubated with the control peptide antigen.
  • IHC - Aquaporin 9 Antibody AG1041-050
    Expression of Aquaporin 9 in rat liver Immunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 antibody (#AG1041) (1:100). Aquaporin 9 (brown staining) is expressed in hepatocytes of the liver parenchyma.
    Hematoxilin is used as the counterstain.
  • IHC - Aquaporin 9 Antibody AG1041-050
    Expression of Aquaporin 9 in rat epididymus Immunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 antibody (#AG1041), (1:100). Aquaporin 9 (brown staining) is expressed in the columnar epithelium of the epididymus (arrows). Hematoxilin is used as the counterstain.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P56627
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 31871 Da
Homology Mouse -13/16 amino acid residues identical.
Additional Information
Gene ID 65054
Other Names Aquaporin-9, AQP-9, Aquaglyceroporin-9, Aqp9
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP-9 (Accession number P56627).ֲ Intracellular, N-terminus.Unlikely to recognize human samples.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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Background

Aquaporin 9 (AQP-9) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.   The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include AQP-1, AQP-2, AQP4, AQP-5, AQP-6 and AQP-8, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.   The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.2   AQP-9 is expressed in several tissues including brain, liver, testis and spleen. In the liver AQP-9 has a central role in glycerol metabolism as demonstrated in experiments using AQP-9 knockout mice: mice deficient in AQP-9 expression showed enhanced plasma levels of glycerol and triglycerides.3   Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular N-terminus domain of the rat AQP-9 channel. Anti-Aquaporin 9 antibody (#AG1041) can be used in western blot and immunohistochemical applications, and recognizes AQP-9 from rat and mouse samples.

References

References 1. Rojek, A.M. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 3609. 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 3. Koyama, Y. et al. (1997) J Biol Chem 272, 30329.

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$ 475.00
$ 575.00
Cat# AG1041-050
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