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Aquaporin 8 Antibody

Affinity purified polyclonal antibody

     
  • WB - Aquaporin 8 Antibody AG1042-050
    Western blot analysis of rat small intestine membranes:
    1. Anti-Aquaporin 8 antibody (#AG1042), (1:200).
    2. Anti-Aquaporin 8 antibody, preincubated with the control peptide antigen.
  • WB - Aquaporin 8 Antibody AG1042-050
    Western blot analysis of rat pancreas membranes:
    1. Anti-Aquaporin 8 antibody (#AG1042), (1:400).
    2. Anti-Aquaporin 8 antibody, preincubated with the control peptide antigen.
  • IHC - Aquaporin 8 Antibody AG1042-050
    Expression of Aquaporin 8 in rat pancreas Immunohistochemical staining of rat pancreas paraffin-embedded sections using Anti-Aquaporin 8 antibody (#AG1042), (1:100). Staining (brown color) is specific for the apical portion of acinar cells in the exocrine pancreas. Hematoxilin is used as the counterstain.
  • IHC - Aquaporin 8 Antibody AG1042-050
    Expression of Aquaporin 8 in rat lung Immunohistochemical staining of rat lung paraffin-embedded sections using Anti-Aquaporin 8 antibody (#AG1042), (1:100). Staining (brown color) is specific for the bronchiolar epithelium. Hematoxilin is used as the counterstain.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P56405
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28055 Da
Homology Unlikely to recognize mouse or human samples.
Additional Information
Gene ID 29172
Other Names Aquaporin-8, AQP-8, Aqp8
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)REIKGKETNMADSYH, corresponding to amino acid residues 14-28 of rat AQP-8 (Accession number P56405). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Background

Aquaporin 8 (AQP-8) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes.1 The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.   The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are permeated primarily by water and include besides AQP-8, AQP-1, AQP-2, AQP4 and AQP-5, and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-3, AQP-7, AQP-9 and AQP-10.   The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.2   AQP-8 is expressed along the gastrointestinal tract including small intestine, colon, pancreas and liver and also in other organs such as testis, lung and kidney.   The physiological function of AQP-8 is not entirely clear but studies suggest that it has an important role in colonic water adsorption and hepatocyte bile formation.3,4 Interestingly, AQP-8 expression is markedly altered in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.5

References

References 1.  Zahn, A. et al. (2007) World J. Gastroenterol. 13, 1687. 2. Laforenza, U. et al. (2005) J. Nutr. 135, 2329. 3. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 4. Ma, T. and Verkman, A.S. (1999) J. Physiol. 517, 317. 5. Koyama, Y. et al. (1997) J Biol Chem 272, 30329

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$ 475.00
$ 575.00
Cat# AG1042-050
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