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Aquaporin 7 Antibody

Affinity purified polyclonal antibody

  • WB - Aquaporin 7 Antibody AG1043-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney membranes: 1, 2. Anti-Aquaporin 7 antibody, (#AG1043), (1:500).
    3, 4. Anti-Aquaporin 7 antibody, preincubated with the control peptide antigen.
  • IHC - Aquaporin 7 Antibody AG1043-025
    Expression of Aquaporin 7 in rat kidney Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 7 antibody (#AG1043), (1:100). A. In the minor calyx, Aquaporin 7 staining (deep red) is specific for epithelial cells of the uroepithelium  B. In the cortical labyrinth, Aquaporin 7 staining is specific for renal tubules while the stronger staining is evident in the brush border (blue arrows). Hematoxilin is used as the counterstain.
  • ICC - Aquaporin 7 Antibody AG1043-025
    Expression of Aquaporin 7 in rat adipocytes Immunocytochemical staining of 3T3-L1 adipocytes using Anti-Aquaporin 7 antibody (#AG1043), (1:800), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red) (A). C. Anti-Aquaporin 7 antibody, preincubated with the control peptide antigen followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B., D. show visible light images of the adipocytes shown on (A) and (C) respectively.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P56403
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28882 Da
Homology Mouse -15/18 amino acid residues identical; human -11/18 amino acid residues identical.
Additional Information
Gene ID 29171
Other Names Aquaporin-7, AQP-7, Aquaglyceroporin-7, Aqp7
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide corresponding to amino acidֲ residues 7-24 of rat AQP-7 (Accession number P56403). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.
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Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.   The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.   The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.   AQP-7 is expressed in ovary, testis, kidney and adipose tissue.   The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and type 2 diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size


References 1. Kuriyama, H.  et al. (1997) Biochem. Biophys. Res. Commun. 241, 53. 2. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 3. Fruhbeck, G. et al. (2006) Trends Pharmacol. Sci. 27, 345.

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Cat# AG1043-025
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