|Calculated MW||28860 Da|
|Homology||Mouse -15/18 amino acid residues identical.|
|Other Names||Aquaporin-6, AQP-6, Aqp6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EPQKKESQTNSEDTEVSV corresponding to amino acidֲ residues 259-276 of rat AQP6.ֲ ֲ (Accession Q9WTY0). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
It has been long known that water must cross biological membranes by means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question was answered. The importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels. Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of six transmembrane domains with intracellular N and C-termini. Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney, particularly in intracellular vesicles in collecting duct intercalated cells. Unlike the other aquaporins, AQP6 was found to allow permeation of anions following activation with acidic pH or Hg2+ ions. Increased expression of AQP6 was shown in models of chronic alkalosis and lithium-induced nephrogenic diabetes insipidus.
References 1. Yasui, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 5808. 2. Ikeda, M. et al. (2002) J. Biol. Chem. 277, 39873. 3. Agre, P. et al. (2002) J. Physiol. 542.1, 3.
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