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Aquaporin 6 Antibody

Affinity purified polyclonal antibody

  • WB - Aquaporin 6 Antibody AG1044-050
    Western blot analysis of rat kidney membranes: 1. Anti-Aquaporin 6 antibody (#AG1044), (1:200).
    2. Anti-Aquaporin 6 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9WTY0
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28860 Da
Homology Mouse -15/18 amino acid residues identical.
Additional Information
Gene ID 29170
Other Names Aquaporin-6, AQP-6, Aqp6
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EPQKKESQTNSEDTEVSV corresponding to amino acidֲ residues 259-276 of rat AQP6.ֲ ֲ (Accession Q9WTY0). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 0.025% NaN3.
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It has been long known that water must cross biological membranes by  means other than simple diffusion. However, it was not until recently, with the discovery of the Aquaporin 1 water channel that this question  was answered. The  importance of this discovery was underlined by the awarding, in 2003, the Nobel Prize in Chemistry to Peter Agre for the discovery of water channels.  Today, eleven mammalian proteins that belong to the Aquaporin family have been identified. The proteins present a conserved structure of  six transmembrane domains with intracellular N and C-termini. Aquaporin 6 (AQP6) appears to be exclusively expressed in the kidney,  particularly in intracellular vesicles in collecting duct intercalated cells.  Unlike the other aquaporins, AQP6 was found to allow permeation of anions  following activation with acidic pH or Hg2+ ions. Increased expression of  AQP6 was shown in models of chronic alkalosis and lithium-induced  nephrogenic diabetes insipidus. 


References 1. Yasui, M. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 5808. 2. Ikeda, M. et al. (2002) J. Biol. Chem. 277, 39873. 3. Agre, P. et al. (2002) J. Physiol. 542.1, 3.

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$ 475.00
$ 575.00
Cat# AG1044-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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