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Aquaporin 2 Antibody

Affinity purified polyclonal antibody

  • WB - Aquaporin 2 Antibody AG1047-025
    Western blot analysis of rat kidney membranes:
    1. Anti-Aquaporin 2 antibody (#AG1047), (1:200).
    2. Anti-Aquaporin 2 antibody, preincubated with a control peptide antigen.
  • IHC - Aquaporin 2 Antibody AG1047-025
    Expression of Aquaporin 2 in rat kidney
    Immunohistochemical staining of rat kidney paraffin embedded section showing the inner medulla using Anti-Aquaporin 2 antibody (#AG1047), (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P34080
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28931 Da
Homology Mouse - identical; human, sheep - 17/18 amino acidresidues identical.
Additional Information
Gene ID 25386
Other Names Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, AQP-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct, Aqp2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RQSVELHSPQSLPRGSKA, corresponding to amino acidֲ residues 254-271 of rat AQP2 (Accession number P34080).ֲ ֲ Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Immunohistochemistry (IH): - Mouse paraffin-embedded kidney sections (1:200) (see Kim, J.I. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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Aquaporin 2 (AQP-2) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.   The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-2 together with AQP-1, AQP-4 and AQP-5 belongs to the first group.1   The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1 AQP-2 expression is largely confined to the kidney, particularly in the renal collecting duct where it performs a key role in water absorption and urine concentration. In fact, mutations in the AQP-2 gene produce hereditary nephrogenic diabetes insipidus, a disorder that results in the excretion of large volumes of urine.2   Under normal conditions, water homeostasis in the kidney is regulated through the anti-diuretic hormone vasopressin. Vasopressin is secreted from the pituitary gland and transported to the kidney through the blood where it binds to its receptor that is mainly expressed in cells of the collecting duct. The activated vasopressing receptor induces an increase in intracellular cAMP and subsequent PKA activation, which phosphorylates AQP-2. This phosphorylation causes the translocation of AQP-2 channels from intracellular vesicles to the cell membrane where it markedly increases water permeability.1, 3, 4


References 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 2. Deen, P.M. et al. (1994) Science 264, 92. 3. Nielsen, S. et al. (2002) Physiol. Rev. 82, 205. 4. Robben, J.H. et al. (2006) Am. J. Physiol. Renal Physiol. 291, F257.

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$ 375.00
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$ 575.00
Cat# AG1047-025
(40 western blots)
Availability: 2-3 weeks
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