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Aquaporin 1 Antibody

Affinity purified polyclonal antibody

  • WB - Aquaporin 1 Antibody AG1048-050
    Western blot analysis of rat kidney membranes: 1. Anti-Aquaporin 1 antibody (AG1048), (1:400).
    2. Anti-Aquaporin 1 antibody, preincubated with the control peptide antigen.
  • IHC - Aquaporin 1 Antibody AG1048-050
    Expression of Aquaporin 1 in rat kidney Immunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 1 antibody (#AG1048), (1:100). AQP1 staining (brown) is present in both distal (DT) and proximal (PT) convoluted tubules in the renal cortex. Note that a weak staining is also present in collecting ducts (CD) while glomeruli (Gl) are negative. Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29972
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 28526 Da
Homology rat, mouse, cattle, sheep - identical; dog - 18/19 amino acidresidues identical; frog (Rana esculenta), toad (Bufo marinus) - 14/19 amino acid residues identical.
Additional Information
Gene ID 358
Other Names Aquaporin-1, AQP-1, Aquaporin-CHIP, Urine water channel, Water channel protein for red blood cells and kidney proximal tubule, AQP1, CHIP28
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KVWTSGQVEEYDLDADDIN corresponding to amino acid residues 242-260 of human AQP1 (accession number P29972). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Immunohistochemistry (IH): - Mouse paraffin-embedded kidney sections (1:100) (see Kim, J.I. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Research Areas
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Aquaporin 1 (AQP-1) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).   The aquaporins can be divided into two functional groups based on their permability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-1 together with AQP-2, AQP-4 and AQP-5 belongs to the first group.1 Little is known about the function of the two newest members, AQP-11 and AQP-12.   The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores.1   AQP-1 is widely expressed in several organs with prominent expression found in kidney, lung, red blood cells intestine and brain. Studies with mice lacking AQP-1 show that the channel has a critical role in urine concentration.2 In addition, AQP-1 expression in tumor cells was shown to contribute to enhanced tumor spread and metastatic potential.3


References 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 2. Ma, T. et al. (1998) J Biol Chem 273, 4296. 3. Hu. J. and Verkman, A.S. (2006) FASEB J. 20, 1892.

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$ 475.00
$ 575.00
Cat# AG1048-050
(40 western blots)
Availability: 2-3 weeks
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