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Prostanoid EP2 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Prostanoid EP2 Receptor (extracellular) Antibody AG1053-025
    Western blot analysis of rat brain lysate (lanes 1 and 4), rat stomach  lysate (lanes 2 and 5) and mouse kidney lysate (lanes 3 and 6): 1-3. Anti-Prostanoid EP2 Receptor (extracellular) antibody (#AG1053), (1:500).
    4-6. Anti-Prostanoid EP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Prostanoid EP2 Receptor (extracellular) Antibody AG1053-025
    Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6): 1-3. Anti-Prostanoid EP2 Receptor (extracellular) antibody (#AG1053), (1:500).
    4-6. Anti-Prostanoid EP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Prostanoid EP2 Receptor (extracellular) Antibody AG1053-025
    Expression of Prostanoid EP2 Receptor in rat cerebellum Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostanoid EP2 Receptor (extracellular) antibody (#AG1053), (1:100). Prostanoid EP2 Receptor staining (red) is detected inPurkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.
  • FC - Prostanoid EP2 Receptor (extracellular) Antibody AG1053-025
    Indirect flow cytometry analysis of live intact human Jurkat T cell leukemia cell line: ___ Unstained cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-Prostanoid EP2 Receptor (extracellular) antibody (#AG1053), (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q62928
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 39772 Da
Homology Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
Additional Information
Gene ID 81752
Other Names Prostaglandin E2 receptor EP2 subtype, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostanoid EP2 receptor, Ptger2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:100
FC~~1:15
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Background

Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors.   There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with extracellular N-terminal tail and intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles in the eye3.   PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4. EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

References

References 1. Campbell, W.B. et al. (1990) The Pharmacological Basis of Therapeutic 8, 600. 2. Davies, P. et al. (1992) Inflammation: Basic Principles and Clinical Correlates 2, 123. 3. Coleman, R.A. et al. (1992) Comprehensive Medicinal Chemistry 3, 123. 4. Ferreri, N.R. et al. (1992) J. Biol. Chem. 267, 9443. 5. Bastien, L. et al. (1994) J. Biol. Chem. 269, 11673.

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Cat# AG1053-025
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