|Calculated MW||43603 Da|
|Homology||Mouse- 11/13 amino acid residues identical; human - 9/13 amino acid residues identical.|
|Other Names||Prokineticin receptor 2, PK-R2, G-protein coupled receptor 73-like 1, G-protein coupled receptor I5E, Prokr2, Gpr73l1, Pkr2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat Prokineticin Receptor 2 (Accession Q8R415). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation. The biological effects of the PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin Receptor 1 (PKR1) and Prokineticin Receptor 2 (PKR2) that belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1 PKR2 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. The distribution of PKR2 is relatively restricted with high expression levels in the brain, spinal cord and dorsal root ganglions, in organs of the reproductive system and in endocrine tissues such as the thyroid, pituitary and adrenal glands.1 The PK2 ligand has been shown to be involved in the regulation of circadian rhythms of physiological and behavioral processes in mammals, probably through signaling via PKR2 which is highly expressed in the suprachiasmatic nucleus (SCN), an area of the brain that controls circadian rhythm processes.2 In addition, loss of function mutations in the PKR2 gene have been associated with Kallmann Syndrome, a condition characterized by idiopathic hypogonadotropic hypogonadism (IHH) in combination with anosmia, a compromised sense of smell.3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat PKR2 receptor. The epitope is specific for PKR2 and will not cross-react with the closely related PKR1 receptor. Anti-Prokineticin Receptor 2 (extracellular) antibody (#AG1055) can be used in Western blot analysis and immunohistochemical applications, and recognizes PKR2 from rat samples.
References 1. Zhou, Q-Y. (2006) Mol. Interv. 6, 330. 2. Cheng, M.Y. et al. (2002) Nature 417, 405. 3. Pitteloud, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104, 17447.
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