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Prokineticin Receptor-1 (extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Prokineticin Receptor-1 (extracellular) Antibody AG1056-050
    Western blot analysis of rat brain membranes: 1. Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056), (1:200).
    2. Anti-Prokineticin Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Prokineticin Receptor-1 (extracellular) Antibody AG1056-050
    Western blot analysis of mouse brain lysate: 1. Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056), (1:200).
    2. Anti-Prokineticin Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Prokineticin Receptor-1 (extracellular) Antibody AG1056-050
    Expression of Prokineticin Receptor 1 in rat DRG Immunohistochemical staining of frozen rat dorsal root ganglion (DRG) sections using Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056), (1:100), followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. PKR1 (red) is expressed in DRG neurons. Cell nuclei are visualized with Hoechst 33342 (blue).
  • IHC - Prokineticin Receptor-1 (extracellular) Antibody AG1056-050
    Expression of Prokineticin Receptor 1 in mouse brain Immunohistochemical staining of mouse olfactory bulb brain sections using Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056), (1:100). A. Green fluorescence reveals intensely stained astrocyte-like cells in the glomeruli (G) and lightly stained astrocyte-like cells in the adjacent layer (arrows).
    B. Merge of images of PKR1 (green), parvalbumin (red, a neuronal marker) and DAPI (blue) nuclear counterstain.
  • FC - Prokineticin Receptor-1 (extracellular) Antibody AG1056-050
    Indirect Flow cytometry analysis in live intact WEHI-231 (mouse B cell lymphoma) cell lines: Black Untsaiined cells + goat-anti-rabbit-Phycoerythrin (PE).
    Black Cells + Prokineticin Receptor 1 (extracellular) antibody (#AG1056), (1:20) + goat-anti-rabbit-PE.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q8R416
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44507 Da
Homology Mouse - 12/15 amino acid residues identical.
Additional Information
Gene ID 192648
Other Names Prokineticin receptor 1, PK-R1, G-protein coupled receptor 73, G-protein coupled receptor ZAQ, Prokr1, Gpr73, Pkr1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)ENTTNTFTDFFSARD, corresponding to amino acid residues 10-24 of rat Prokineticin receptor 1 (Accession Q8R416). Extracellular, N-terminus.Unlikely to recognize human samples.
Dilution WB~~1:200-1:2000
IHC~~1:100
FC~~1:20
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.   The biological effects of the PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin Receptor 1 (PKR1) and Prokineticin Receptor 2 (PKR2) that belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1   PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described. 1   PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain particularly in olfactory regions as well as in dorsal root ganglion (DRG) neurons.   The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2 In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3   Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat PKR1 receptor. The epitope is specific for PKR1 and will not cross-react with the closely related PKR2 receptor. Anti-Prokineticin Receptor 1 (extracellular) antibody (#AG1056) can be used in Western blot analysis, immunohistochemical and flow cytometry applications, and recognizes PKR1 from rat and mouse samples.

References

References 1. Zhou, Q-Y. (2006) Mol. Interv. 6, 330. 2. Negri, L. et al. (2006) J. Neurosci. 26, 6716. 3. Dorsch, M. et al. (2005) J. Leucoc. Biol. 78, 426.

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$ 475.00
$ 575.00
Cat# AG1056-050
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