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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Protease-activated Receptor-2 Antibody   

Protease-activated Receptor-2 Antibody

Affinity purified polyclonal antibody

  • WB - Protease-activated Receptor-2 Antibody AG1058-025
    Western blot analysis of human breast adenocarcinoma MDA-MB-231(lanes 1 and 4) and MDA-MB-468 (lanes 2 and 5), and human lung small cell carcinoma NCI-H526 (lanes 3 and 6) cell lines: 1-3. Anti-Protease-Activated Receptor-2 antibody (#AG1058), (1:600).
    4-6. Anti-Protease-Activated Receptor-2 antibody, preincubated with the control peptide antigen.
  • WB - Protease-activated Receptor-2 Antibody AG1058-025
    Western blot analysis of rat lung (lanes 1 and 3) and liver (lanes 2 and 4) lysates: 1, 2. Anti-Protease-Activated Receptor-2 antibody (#AG1058), (1:400).
    3, 4. Anti-Protease-Activated Receptor-2 antibody, preincubated with the control peptide antigen.
  • WB - Protease-activated Receptor-2 Antibody AG1058-025
    Western blot analysis of mouse kidney lysates: 1. Anti-Protease-Activated Receptor-2 antibody (#AG1058), (1:400).
    2. Anti-Protease-Activated Receptor-2 antibody, preincubated with the control peptide antigen.
  • WB - Protease-activated Receptor-2 Antibody AG1058-025
    Western blot analysis of rat dorsal root ganglion lysates: 1. Anti-Protease-Activated Receptor-2 antibody (#AG1058), (1:200).
    2. Anti-Protease-Activated Receptor-2, preincubated with the control peptide antigen.
  • ICC - Protease-activated Receptor-2 Antibody AG1058-025
    Expression of PAR-2 in human prostate carcinoma cell line Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human PC-3 cells. A. Cells were stained with Anti-Protease-Activated Receptor-2 antibody (#AG1058), (1:500), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (orange). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merge of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63645
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44441 Da
Homology Mouse -13/15 amino acid residues identical; human 11/15 amino acid residues residues identical.
Additional Information
Gene ID 116677
Other Names Proteinase-activated receptor 2, PAR-2, Coagulation factor II receptor-like 1, Thrombin receptor-like 1, F2rl1, Par2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KRMQISLTSNKFSRK, corresponding to amino acid residues 368-382 of rat PAR-2 (Accession Q63645). Intracelluar, C- terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Protease-activated receptor 2 (PAR-2) belongs to a family of four G protein-coupled receptors (PAR-1 - 4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3   Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin, as are PAR-3 and PAR-4.1-3 PAR-2 is the only PAR family receptor that is activated by trypsin and not by thrombin. PAR-2 can be also cleaved and activated by other proteases such as tryptase, membrane-type serine protease 1, protease 3, and others.1-3   The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby “disarming” the PAR.1-3   PAR-2-mediated intracellular signaling has not been clearly elucidated, but activators of PAR-2 induce generation of IP3 and mobilization of intracellular Ca2+. Activation of the ERK1/2 and NF-kB intracellular pathways following PAR-2 activation has been also described.1-3   Tissue distribution of PAR-2 is wide with the highest expression levels found in pancreas, small intestine, liver and kidney. In addition, PAR-2 expression was observed in dorsal root ganglion neurons, smooth muscle cells and endothelial cells.   The physiological role of PAR-2 is not clearly understood, however studies using PAR-2 knockout mice have suggested roles in the cardiovascular, pulmonary and gastrointestinal systems. Several studies suggest that PAR-2 plays a central role in inflammatory diseases and nociceptive pain transduction; hence PAR-2 blockers have been proposed to be useful in the therapeutic control of inflammation and pain.1-3   Abgent is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminus of the rat protease-activated receptor-2. Anti-Protease-activated Receptor-2 antibody (#AG1058) can be used in western blot analysis and immunocytochemical applications, and has been tested in rat, mouse and human samples.


References 1. MacFarlane, S.R. et al. (2001) Pharmacol. Rev. 53, 245. 2. Hollenberg, M.D. and Compton, S.J. (2002) Pharmacol. Rev. 54, 203. 3. Ossovskaya, V.S. and Bunnett, N.W. (2004) Physiol. Rev. 84, 579.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1058-025
(40 western blots)
Availability: 2-3 weeks
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