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P2X3 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - P2X3 (extracellular) Antibody AG1060-025
    Western blot analysis of rat DRG: 1. Anti-P2X3 Receptor (extracellular) antibody (#AG1060), (1:200).
    2. Anti-P2X3 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - P2X3 (extracellular) Antibody AG1060-025
    Expression of P2X3 Receptor in rat DRG Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-P2X3 Receptor (extracellular) antibody (#AG1060), followed by anti-rabbit-AlexaFluor-594 secondary antibody.  P2X3 Receptor staining (red) appears in neuronal cell bodies. DAPI was used as the counter stain (blue).
    A. X20 magnification. B. X40 magnification.
  • ICC - P2X3 (extracellular) Antibody AG1060-025
    Expression of P2X3 Receptor in rat PC12 cells Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining with Anti-P2X3 Receptor (extracellular) antibody (#AG1060), (1:50, red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI was used as the counter stain (blue). C. Merge images of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49654
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44392 Da
Homology Mouse-identical; human - 13/15 amino acid residues identical.
Additional Information
Gene ID 81739
Other Names P2X purinoceptor 3, P2X3, ATP receptor, Purinergic receptor, P2rx3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 Receptor (Accession P49654). Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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The P2X3 receptor belongs to the ligand-gated ion channel P2X family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP 1-3. All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels4,5. The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals6,7. Involvement of ATP in the mechanism of chronic pain has been also suggested7,8. P2X3 receptor is now becoming a possible target for the development of pain therapeutics.   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat P2X3 receptor. Anti-P2X3 Receptor (extracellular) antibody (#AG1060) can be used in western blot immunohistochemical and immunocytochemical applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.


References 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Kage, K. et al. (2002) Exp.Brain Res. 147, 511. 7. Aoki, Y. et al. (2003) Brain Res. 989, 214. 8. Burnstock, G. (2000) Br. J. Anaesth. 84, 476.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1060-025
(40 western blots)
Availability: 2-3 weeks
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