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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   P2X2 Receptor (extracellular) Antibody   

P2X2 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - P2X2 Receptor (extracellular) Antibody AG1061-050
    Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and PC12 (lanes 3 and 6): 1-3. Anti-P2X2 Receptor (extracellular) antibody (#AG1061), (1:200).
    4-6. Anti-P2X2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - P2X2 Receptor (extracellular) Antibody AG1061-050
    Expression of P2X2 in rat PC12 cells Immunocytochemical staining of intact living rat pheochromocytoma cell line (PC12). A. Extracellular staining of cells with Anti-P2X2 Receptor (extracellular) antibody (#AG1061), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Staining of cells with DAPI used as the counterstain. C. Merged images of A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49653
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 52617 Da
Homology Rat, mouse - 13/14 amino acid residues identical. Will not recognize human P2X2.
Additional Information
Gene ID 114115
Other Names P2X purinoceptor 2, P2X2, ATP receptor, Purinergic receptor, P2rx2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653 ). Extracellular.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1-2 µg peptide per 1 µg antibody.
Research Areas
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The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.   The structure and function of the P2X receptors, which were mainly investigated using in vitro models, show their involvement in synaptic communication, cell death, and differentiation.   Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex4-9.   The various mammalian P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system4. The P2X2 receptor subtype has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes. mRNA of the P2X1, P2X2, P2X3, and P2X6 receptors is found in neurons of dorsal root ganglia (DRG), trigeminal, and nodose ganglia where heteromeric P2X2/P2X3 receptors are found10.   Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat P2X2 Receptor. Anti-P2X2 Receptor (extracellular) antibody (#AG1061) can be used in western blot and immunocytochemistry applications. It has been designed to recognize P2X2 Receptor from mouse and rat samples. It will not recognize human P2X2.


References 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347. 10. Chizh, B.A. and Illes.P. (2001) Pharmacol. Rev.  53, 553.

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$ 475.00
$ 575.00
Cat# AG1061-050
(40 western blots)
Availability: 2-3 weeks
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