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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   P2X1 Receptor (extracellular) Antibody   

P2X1 Receptor (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - P2X1 Receptor (extracellular) Antibody AG1062-050
    Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates: 1, 2. Anti-P2X1 Receptor (extracellular) antibody (#AG1062),  (1:200).
    3, 4. Anti-P2X1 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - P2X1 Receptor (extracellular) Antibody AG1062-050
    Expression of P2X1 in human SH-SYS5 cells Immunocytochemical staining of human intact living SH-SYS5 cells. A. Extracellular staining of cells with Anti-P2X1 (extracellular) antibody (#AG1062), (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. B. Nuclear staining DAPI as the counterstain. C. Merged images of A and B.
  • IHC - P2X1 Receptor (extracellular) Antibody AG1062-050
    Expression of P2X1 in mouse cerebellum Immunohistochemical staining of mouse cerebellum using Anti-P2X1 Receptor (extracellular) antibody (#AG1062). A. Most of P2X1 labeling (green) appears in fine processes in the molecular layer (MOL) and in Purkinje cells (arrows show examples). B. DAPI is used as the counterstain (blue). C. Merge of A and B.
  • FC - P2X1 Receptor (extracellular) Antibody AG1062-050
    Indirect flow cytometry analysis of MEG-O1 cells: ___ Unstained cells.
    ___ Cells + Anti-P2X1 Receptor (extracellular) antibody (#AG1062), (10 µg antibody/1x106 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51575
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44980 Da
Homology Rat, mouse - 11/14 amino acid residues identical.
Additional Information
Gene ID 5023
Other Names P2X purinoceptor 1, P2X1, ATP receptor, Purinergic receptor, P2RX1, P2X1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575 ). Extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations ( 0 )


The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.   Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned1-3. All P2X receptor subtypes share the same structure of intracellular N- and C-termini two membrane-spanning domains and a large extracellular loop.   All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex4-9.   The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous systems, while the P2X7 receptor is predominantly found in cells of the immune system4. The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis3,4,10,11.


References 1. Prasad, M. et al. (2001) J. Physiol. 537, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347. 10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182. 11. Oury, C. et al. (2003) Blood 101, 3969.

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$ 475.00
$ 575.00
Cat# AG1062-050
(40 western blots)
Availability: 2-3 weeks
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