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P2Y14 Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - P2Y14 Receptor (extracellular) Antibody AG1064-025
    Western blot analysis of lysate from rat heart tissue lysate, using P2RY14 Antibody(Cat. #AG1064). AG1064 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • FC - P2Y14 Receptor (extracellular) Antibody AG1064-025
    Flow cytometric analysis of Jurkat cells using Park7 (DJ-1) Antibody(green, Cat#AG1064) compared to an isotype control of rabbit IgG(blue). AG1064 was diluted at 1:40 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - P2Y14 Receptor (extracellular) Antibody AG1064-025
    Western blot analysis of rat heart membranes (lanes 1,4), rat brain (lanes 2,5) and rat lung lysates (lanes 3,6): 1-3. Anti-P2Y14 Receptor (extracellular) antibody, (#AG1064), (1:200). 4-6. Anti-P2Y14 Receptor (extracellular) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15391
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 38971 Da
Homology Rat, mouse - 15/17 amino acid residues identical.
Additional Information
Gene ID 9934
Other Names P2Y purinoceptor 14, P2Y14, G-protein coupled receptor 105, UDP-glucose receptor, P2RY14, GPR105, KIAA0001
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CIELKSELGRKWHKASN, corresponding to amino acid residues 172-188 of human P2Y14 (Accession# Q15391). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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P2Y14 belongs to the P2Y receptor family that is part of the G-protein coupled receptor superfamily. To date eight functional mammalian P2Y receptors have been identified: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13 and P2Y14. The P2Y receptor family mediates the effects of extracellular nucleotides such as ATP, ADP, UTP and in the case of P2Y14 UDP-glucose. UDP-glucose as the other nucleotides can be released to the extracellular milieu as the result of cell damage or by regulated non-lytic mechanisms. P2Y14 is expressed in several tissues including brain, lung and several cells of hematopoietic origin such as T lymphocytes and dendritic cells. The exact function of this receptor in the different tissues has not been clearly established but an involvement in chemotaxis, maturation of immature dendritic cells and neuroimmune regulation has been suggested. Abgent is pleased to offer a highly specific antibody directed against a conserved epitope in the second extracellular loop of the human P2Y14 receptor. Anti-P2Y14 (extracellular) antibody (#AG1064) can be used in Western blot analysis, and will recognize human and mouse samples.


1. Chambers, J.K. et al. (2000) J. Biol. Chem. 275, 10767.
2. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
3. Skelton, L. et al. (2003) J. Immunol. 171, 1941.

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$ 247.00
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$ 475.00
Cat# AG1064-025
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