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P2Y13 Receptor Antibody

Affinity purified rabbit polyclonal antibody

  • WB - P2Y13 Receptor Antibody AG1065-050
    Western blot analysis of rat brain membranes: 1. Anti-P2Y13 Receptor antibody (AG1065), (1:200). 2. Anti-P2Y13 Receptor antibody, preincubated with the control peptide antigen.
  • IHC - P2Y13 Receptor Antibody AG1065-050
    Expression of P2Y13 in rat testis Immunohistochemical staining of paraffin embedded rat epidydimus section using Anti-P2Y13 Receptor antibody (#AG1065), (1:50). Strong and high specific staining of the epithelial cells is shown. Universal Immuno-alkaline-phosphatase kit followed by New Fuchsin Substrate was used for color reaction (red). Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BPV8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 40789 Da
Homology Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical.
Additional Information
Gene ID 53829
Other Names P2Y purinoceptor 13, P2Y13, G-protein coupled receptor 86, G-protein coupled receptor 94, P2RY13, GPR86, GPR94
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2Y13 (Accession Q9BPV8). 2nd Intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Immunocytochemistry (IC): - Human fibroblasts (1:25) (see Pinheiro, A.R. et al. (2013) in Product Citations). - Mouse hippocampal neuronal culture (see del Puerto, A. et al. (2011) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (10 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions. A new member of this family was recently identified, the P2Y13 receptor.1,2 The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gai class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1-3 Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.4 P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.5


1. Communi, D. et al. (2001) J. Biol. Chem. 276, 41479.
2. Zhang, F.L. et al. (2002) J. Pharmacol. Exp. Ther. 301, 705.
4. Wang, L. et al. (2005) Circ. Res. 96, 189.
5. Wirkner, K. et al. (2004) Br. J. Pharmacol. 141, 141.

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$ 475.00
$ 575.00
Cat# AG1065-050
(40 western blots)
Availability: 2-3 weeks
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