|Application ||WB, IHC, FC, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||40789 Da|
|Homology||Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical.|
|Other Names||P2Y purinoceptor 13, P2Y13, G-protein coupled receptor 86, G-protein coupled receptor 94, P2RY13, GPR86, GPR94|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2Y13 (Accession Q9BPV8). 2nd Intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Immunocytochemistry (IC): - Human fibroblasts (1:25) (see Pinheiro, A.R. et al. (2013) in Product Citations). - Mouse hippocampal neuronal culture (see del Puerto, A. et al. (2011) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (10 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions. A new member of this family was recently identified, the P2Y13 receptor.1,2 The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gai class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1-3 Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.4 P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.5
1. Communi, D. et al. (2001) J. Biol. Chem. 276, 41479.
2. Zhang, F.L. et al. (2002) J. Pharmacol. Exp. Ther. 301, 705.
4. Wang, L. et al. (2005) Circ. Res. 96, 189.
5. Wirkner, K. et al. (2004) Br. J. Pharmacol. 141, 141.
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