|Application ||WB, IHC, FC, ICC|
|Calculated MW||40345 Da|
|Homology||Rat, mouse - 16/18 amino acid residues identical.|
|Other Names||P2Y purinoceptor 11, P2Y11, P2RY11|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NATAAPKPSEPQSRELS, corresponding to amino acid residues 357-373 of human P2Y11 (Accession Q96G91). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Human derived keratinocytes (HaCaT cells), (1:200), (see Ishimaru, M. et al. (2013) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (6 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.9 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The human P2Y11 receptor is unique among the P2Y receptors since it is dually coupled to phospholipase C and adenylyl cyclase.4-5 P2Y11 receptor is highly pressed in spleen and for much lower extent at platelets. Expression of P2Y11 receptor was also shown in endothelial cells.6 In granulocytes HL-60), P2Y11 receptor is thought to have a role in granulocytic differentiation.4,7
1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
4. Communi, D. et al. (2000) FEBS Letters 475, 39.
5. White, P.J. et al. (2003) Mol. Pharmacol. 63, 1356.
6. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
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