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Affinity purified polyclonal antibody
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Application 
| WB |
|---|---|
| Primary Accession | P51579 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 42451 Da |
| Homology | Mouse - 15/17 amino acid residues identical;human - 14/17 amino acid residues identical. |
| Gene ID | 25041 |
|---|---|
| Other Names | P2X purinoceptor 6, P2X6, ATP receptor, P2XM, Purinergic receptor, Purinergic receptor P2X-like 1, P2rx6, P2rxl1 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide (C)RTKYEEARAPKATTNSA, corresponding to amino acid residues 363-379 of rat P2X6 Receptorֲ (accession number P51579). Intracellular, C-terminus. |
| Dilution | WB~~1:200-1:2000 |
| Peptide Confirmation | Confirmed by mass-spectrography and amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.7 mg/ml. |
| Buffer After Reconstitution | Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl water. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. P2X receptors are involved in synaptic communication and in diseases of the nervous system. The P2X receptors family consist of at least seven isoforms: P2X1-P2X7.1,2,3 All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9 The P2X6 receptor assembles to a heteromultimer with two other P2X subunits, P2X2 and P2X4. The P2X6 receptor is extensively expressed throughout the central nervous system.7
References
References 1. Prasad, M.et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience. 115, 425. 3. Ashcroft, F.M. et al. (2000) J Ion Channels and Disease Ed 1. 4. Khakh, B.S. et al. (2001) Pharmacol Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Le, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347.
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