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P2Y12 Receptor Antibody

Affinity purified polyclonal antibody

  • WB - P2Y12 Receptor Antibody AG1068-025
    Western blot analysis of rat brain membranes (1, 2) and human platelets (3, 4):  1, 3. Anti-P2Y12 Receptor antibody (#AG1068), (1:200).   
    2, 4. Anti-P2Y12 Receptor antibody, preincubated with the control peptide antigen.
  • IHC - P2Y12 Receptor Antibody AG1068-025
    Expression of P2Y12 in mouse brain Immunohistochemical staining of P2Y12 (red) in mouse brain using Anti-P2Y12 Receptor antibody (#AG1068). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H244
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 39439 Da
Homology Rat, mouse - 16/18 amino acid residues identical.
Additional Information
Gene ID 64805
Other Names P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999, P2RY12, HORK3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KTTRPFKTSNPKNLLGAK,ֲ  corresponding to amino acid residues 125-142 of human P2Y12 receptor. (Accession Q9H244). Intracellular, 2nd Cytoplasmic loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Immunocytochemistry (IC): - Human fibroblasts (1:100) (see Pinheiro, A.R. et al. (2013) in Product Citations). - Rat astrocytes (1:50) (see Carrasquero, L.M.G. et al. (2005) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (8 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The P2Y receptors belong to the G-protein coupled receptors superfamily.   They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP AND UDPA. Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3  The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.    The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4


References 1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. 2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. 3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. 4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1068-025
(40 western blots)
Availability: 2-3 weeks
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