|Reactivity||Human, Mouse, Rat|
|Calculated MW||39439 Da|
|Homology||Rat, mouse - 16/18 amino acid residues identical.|
|Other Names||P2Y purinoceptor 12, P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999, P2RY12, HORK3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KTTRPFKTSNPKNLLGAK,ֲ corresponding to amino acid residues 125-142 of human P2Y12 receptor. (Accession Q9H244). Intracellular, 2nd Cytoplasmic loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunocytochemistry (IC): - Human fibroblasts (1:100) (see Pinheiro, A.R. et al. (2013) in Product Citations). - Rat astrocytes (1:50) (see Carrasquero, L.M.G. et al. (2005) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (8 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP AND UDPA. Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4
References 1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. 2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. 3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. 4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
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