|Application ||WB, IHC, FC, IP, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42322 Da|
|Homology||Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.|
|Other Names||P2Y purinoceptor 1, P2Y1, ATP receptor, Purinergic receptor, P2ry1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2Y1. (Accession P49651, P47900). 3rd intracellular loop (i3) between TM5 and TM6 domains.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse spinal cord astrocyte lysate (1:200) (see Suadicani, S.O. et al. (2003) in Product Citations). Immunoprecipitation (IP): - Rat cortical neuron lysate (4 μg) (see Siow, N.L. et al. (2010) in Product Citations). Immunohistochemistry (IH): - Human eye tissue (see Fries, J.E. et al. (2005) in Product Citations). Immunocytochemistry (IC): - Human fibroblasts (1:50) (see Pinheiro, A.R. et al. (2013) in Product Citations). - Rat primary hippocampal neurons (1:100) (see Filippov, A.K. et al. (2006) in Product Citations). Indirect flow cytometry (IFC): - Rat osteoclasts (6 μg/ml) (see Alvarenga, E.C. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1 The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4 P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
4. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
5. Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
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