|Reactivity||Human, Mouse, Rat|
|Calculated MW||68388 Da|
|Homology||Human, rat- identical; bovine - 14/17 amino acid residues identical.|
|Other Names||P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7, P2x7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouseֲ P2X7 Receptor (Accession number Q9Z1M0). Extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Application Details||Western blot analysis (WB): - Equine hoof lysate (1:200) (see Zamboulis, D.E. et al. (2013) in Product Citations). - Human mesenchymal stem cells (hMSCs) (see Sun, D. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Equine palmar digital artery and Schwann cells (1:200) (see Zamboulis, D.E. et al. (2013) in Product Citations). - Primate retina (1:500) (see Gu, B.J. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Human mesenchymal stem cells (hMSCs) (1:100) (see Sun, D. et al. (2013) in Product Citations). - Mouse P2X7 transfected in CHO cells (1:500). (see Adriouch, S. et al. (2005) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3 Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
References 1. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 2. Kim, M. et al. (2001) EMBO J. 20, 6347. 3. Chizh, B.A. and Illes, P. (2001) Pharmacol. Rev. 53, 553. 4. Nihei, O.K. et al. (2000) Mem.Inst.Oswaldo Cruz. 95, 415.
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