|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||40894 Da|
|Homology||Mouse - 13/14 amino acid residues identical.|
|Other Names||P2Y purinoceptor 4, P2Y4, P2ry4, P2y4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2Y4 (Accession O35811). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.9 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1 The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4 The P2Y4-receptor is highly expressed in the placenta and with lower levels at lung and vascular smooth muscle.5 The human P2Y4 receptor is highly selective to UTP while rat P2Y4 receptor is equally activated by both UTP and ATP and also by other triphosphate nucleotides such as Ap4A.5 Recently it has been shown that P2Y4 receptor is involved in differentiation and cell death of human neuroblastoma SH-SY5Y cells.6 Abgent is pleased to offer a highly specific antibody directed against the C terminus domain of the rat P2Y4 receptor. Anti-P2Y4 Receptor antibody (#AG1072) can be used in western blot analysis, immunoprecipitation, indirect flow cytometry, immunocytochemistry and immunohistochemistry, and recognizes P2Y4 in rat samples.
References 1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841. 2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. 3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. 4. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. 5. Von Kugelgen,I. and Wetter, A. (2000) Naunyn-Schmiedebergs Arch Pharmacol. 362, 310. 6. Cavaliere, F. et al. (2005) Neurobiol. Dis. 18,100.
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