|Calculated MW||51479 Da|
|Homology||Mouse- 13/18 amino acid residues identical.|
|Other Names||P2X purinoceptor 5, P2X5, ATP receptor, Purinergic receptor, P2rx5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 ofֲ rat P2X5 Receptor (Accessionֲ P51578). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and by massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The P2X5 receptor (P2X5R) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.1,2,3 All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The P2X5R forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.4,6 The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia. The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland. Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.7-10 We are pleased to offer a new antibody to our growing line of ligand-gated ion channel P2X antibodies, Anti-P2X5 Receptor antibody.
References 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Torres, G.E. et al. (1998) Mol. Pharmacol. 54, 989. 7. Greig, A.V. et al. (2003) J. Invest. Dermatol. 121, 1145. 8. Greig, A.V. et al. (2003) J. Invest. Dermatol. 120, 1007. 9. Slater, M. et al. (2000) Histochem. J. 32, 571. 10. Afework, M. and Burnstock, G. (2000) Int. J. Devl. Neuroscience 18, 515.
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