|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||68392 Da|
|Homology||Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.|
|Other Names||P2X purinoceptor 7, P2X7, ATP receptor, P2Z receptor, Purinergic receptor, P2rx7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 Receptorֲ (Accession Q64663). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat hippocampus lysate (1:1000) (see Yu, Q. et al. (2013) in Product Citations). - Mouse lumbar spinal cord protein lysates (1:500) (see Apolloni, S. et al. (2013) in Product Citations). Also tested in P2X7-/- mice. - Mouse astrocyte culture lysate (1:500) (see Okuda, H. et al. (2013) in Product Citations). - Rat PC12 cells (1:1000) (see Xu, H. et al. (2013) in Product Citations). - Mouse cerebral synaptosomes (see Naviaux, R.K. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Mouse brain, lung and submandibular glands (see Sim, J.A. et al. (2004) in Product Citations). Immunohistochemistry (IH): - Rat brain sections (1:1000) (see Yu, Q. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat PC12 cells (see Xu, H. et al. (2013) in Product Citations). - Mouse pancreatic stellate cells (PSCs), (1:100) (see Haanes, K.A. et al. (2012) in product Citations). - Human MG63 and SaOS2 osteosarcoma cell lines (see Alqallaf, S.M. et al. (2009) in Product Citations.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned. The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3
References 1. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 2. Kim, M. et al. (2001) EMBO J. 20, 6347. 3. Chizh, B.A. and Illes, P. (2001) Pharmacol. Rev. 53, 553.
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