|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||44957 Da|
|Homology||Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.|
|Other Names||P2X purinoceptor 1, P2X1, ATP receptor, Purinergic receptor, RP-2 protein, P2rx1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 Receptorֲ (Accession P47824). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Equine palmar digital artery (1:200) (See Zamboulis, D.E. et al. (2013) in Product Citations). - Rat detrusor cell lysate (1:100) (see Uvin, P. et al. (2013) in Product Citations). Immunoprecipitation (IP): - HEK-293 transfected cells (see Agboh, K.C. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Equine palmar digital artery and DRGs (1:200) (see Zamboulis, D.E. et al. (2013) in Product Citations). - Mouse vas deferens (see White, W.C. et al. (2013) in Product Citations). Also tested in KO animals. - Mouse mesenteric arteries (see Vial, C. and Evans, R.J. (2002) in Product Citations). Immunocytochemistry (IC): - Human Jurkat and CD4+ T cells (see Woerhle, T. et al. (2010) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation. Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop. All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9 The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis.3,4,10,11
References 1. Prasad, M. et al. (2001) J. Physiol. 537, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347. 10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182. 11. Oury, C. et al. (2003) Blood 101, 3969.
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