Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.P2X1 Receptor Antibody
Affinity purified polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IP |
|---|---|
| Primary Accession | P47824 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 44957 Da |
| Homology | Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical. |
| Gene ID | 25505 |
|---|---|
| Other Names | P2X purinoceptor 1, P2X1, ATP receptor, Purinergic receptor, RP-2 protein, P2rx1 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 Receptorֲ (Accession P47824). Intracellular, C-terminus. |
| Dilution | WB~~1:200-1:2000 |
| Peptide Confirmation | Confirmed by amino acid analysis. |
| Application Details | Western blot analysis (WB): - Equine palmar digital artery (1:200) (See Zamboulis, D.E. et al. (2013) in Product Citations). - Rat detrusor cell lysate (1:100) (see Uvin, P. et al. (2013) in Product Citations). Immunoprecipitation (IP): - HEK-293 transfected cells (see Agboh, K.C. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Equine palmar digital artery and DRGs (1:200) (see Zamboulis, D.E. et al. (2013) in Product Citations). - Mouse vas deferens (see White, W.C. et al. (2013) in Product Citations). Also tested in KO animals. - Mouse mesenteric arteries (see Vial, C. and Evans, R.J. (2002) in Product Citations). Immunocytochemistry (IC): - Human Jurkat and CD4+ T cells (see Woerhle, T. et al. (2010) in Product Citations). |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl water. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 1 µg peptide per 1 µg antibody. |
| Formulation | Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abgent.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation. Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop. All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9 The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis.3,4,10,11
References
References 1. Prasad, M. et al. (2001) J. Physiol. 537, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Lê, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347. 10. Burnstock, G. (2001) Trends Pharmacol. Sci. 22, 182. 11. Oury, C. et al. (2003) Blood 101, 3969.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Ordering Information
Other Products
Shipping Information
