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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   KV7.5 Antibody   

KV7.5 Antibody

Affinity purified polyclonal antibody

  • WB - KV7.5 Antibody AG1077-050
    Western blot analysis of rat hippocampus lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) an rat brain lysate (lanes 3 and 6): 1-3. Anti-Kv7.5 antibody (#AG1077), (1:200).
    4-5. Anti-Kv7.5 antibody, preincubated with the control peptide antigen.
  • IHC - KV7.5 Antibody AG1077-050
    Expression of Kv7.5 in rat hippocampus Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Kv7.5 antibody (#AG1077), (1:100). A. Kv7.5 (green) is expressed in hilar cells of the hippocampal dentate gyrus. B. Double-staining of Kv7.5 (green) with a neuronal marker (“Milli-Mark” from Millipore, red) reveals partial localization with hilar interneurons (arrow points at an example).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JK45
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 102258 Da
Homology Human, rat - identical.
Additional Information
Gene ID 226922
Other Names Potassium voltage-gated channel subfamily KQT member 5, KQT-like 5, Potassium channel subunit alpha KvLQT5, Voltage-gated potassium channel subunit Kv75, Kcnq5
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse Kv7.5 (Accession Q9JK45). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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KCNQ genes encode members of the KV7 K+ channels. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. Five members belong to this family (KV7.1-KV7.5) of which KV7.2 to KV7.5 are expressed in the nervous system1-3. KV7.5 is also expressed in visceral smooth muscle4 and skeletal muscle5. These channels form subunits of the low threshold voltage gated K+ channel. KV7.2 and KV7.3 mostly form heteromers to form the M current. KV7.5 is known to contribute to this current1.


References 1. Brown, D.A. and Passmore, G.M. (2009) Br. J. Pharmacol. 156, 1185. 2. Jentsch, T.J. (2000) Nat. Rev. Neurosci. 1, 21. 3. Robbins, J. (2001) Pharmacol. Ther. 90, 1. 4. Jensen, H.S. et al. (2005) Brain Res. Mol. Brain Res. 139, 52. 5. Lerche, C. et al. (2000) J. Biol. Chem. 275, 22395.

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$ 475.00
$ 575.00
Cat# AG1077-050
(40 western blots)
Availability: 2-3 weeks
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