|Reactivity||Human, Mouse, Rat|
|Calculated MW||29471 Da|
|Homology||Human, mouse- 11/12 amino acid residues identical.|
|Other Names||Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, DREAM, Kv channel-interacting protein 3, KChIP3, Kcnip3, Csen, Dream, Kchip3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3 (Accession Q9JM47). Cytoplasmic, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage gate K+ (KV) channels form functional entities by the assembly of four a subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influence the trafficking of the channels to the cell’s plasma membrane1. KChIPs (KV Channel Interacting Proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region is differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP2, 3 and 4 strongly regulate the activity of the KV4 channel family in cortical pyramidal neurons5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts6. KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart and in the brain4-7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat KChIP3. Anti-KChIP3 antibody (#AG1080) can be used for western blot analysis. It has been designed to recognize KChIP3 from human mouse and rat samples.
References 1. Wang, K. (2008) Neurochem. Res. 33, 1964. 2. Shibata, R. et al. (2003) J. Biol. Chem. 278, 36445. 3. An, W.F. et al. (2000) Nature 403, 553. 4. Holmqvist, M.H. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 1035. 5. Norris, A.J. et al. (2010) J. Neurosci. 30, 13644. 6. Xia, K. et al. (2010) Molec. Brain 3, 1. 7. Xiong, H. et al. (2004) Brain Res. Mol. Brain Res. 128, 203.
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