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Affinity purified polyclonal antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | A2BDX4 |
| Reactivity | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 58176 Da |
| Homology | Rat - identical; human - 11/13 amino acid residues identical. |
| Gene ID | 241794 |
|---|---|
| Other Names | Potassium voltage-gated channel subfamily G member 1, Voltage-gated potassium channel subunit Kv61, Kcng1 |
| Related products for control experiments | Control peptide antigen (supplied with the antibody free of charge). |
| Target/Specificity | Peptide (C)RRKPSTGNSYLDK, corresponding to amino acid residues 325- 337 of mouse Kv6.1 (Accession A2BDX4). 2nd extracellular loop. |
| Dilution | WB~~1:200-1:2000 IHC~~1:200 |
| Peptide Confirmation | Confirmed by mass-spectrography and amino acid analysis. |
| Format | Affinity purified antibody, lyophilized powder |
| Reconstitution | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| Antibody Concentration After Reconstitution | 0.8 mg/ml. |
| Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Storage After Reconstitution | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| Control Antigen Storage Before Reconstitution | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| Control Antigen Reconstitution | 100 µl DDW. |
| Control Antigen Storage After Reconstitution | -20ºC. |
| Preadsorption Control | 2 µg peptide per 1 µg antibody. |
| Formulation | Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Voltage-gated K+ channels are transmembrane proteins consisting of four α-subunits arranged in a radially symmetric fashion around a central aqueous pore. Each α-subunit consists of six transmembrane segments (S1-S6) with cytoplasmic NH2- and COOH-termini. In mammals, a large family of genes encodes α-subunits: KV1 (homologous to Drosophila Shaker), KV2 (Shab), KV3 (Shaw), KV4 (Shal), KV5, KV6, and KV91. The sparsely populated KV channel subfamilies KV5 and KV6 each contain one member, KV5.1 and KV6.12. KV6 channels are electrically silent and no functional role has been suggested for these channels3. A recent study found that KV6.1 forms functional channels specifically with KV2.13. It was also found that KV2.1 and KV6.1 mRNAs colocalize to the piriform cortex, hippocampus, dendate gyrus and olfactory tubercle in the brain4 and to SA node, atria and ventricle in the heart5. This suggests that KV6.1 may be physiologically important in generating an increased number of KV channel phenotypes even though it does not itself form functional homomultimeric channels3. Another study found that KV6.1 regulates the kinetics of KV2.2 channels. KV2.1/6.1 coexpression results in channels with markedly slower inactivation during strong depolarization but faster inactivation at intermediate potentials1.
References
References 1. Kramer J.W. et al. (1998) Am. J. Physiol. 274, C1501. 2. Chandy, K.G., et al. (1995) Ligand- and Voltage-Gated Ion Channels, R.A. North, Ed. 1. 3. Post, M.A. et al. (1996) FEBS Lett. 399, 177. 4. Drewe, J.A. et al. (1992) J. Neurosci. 12, 538. 5. Dixon, J.E. et al. (1994) Circ. Res. 75, 252.
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