|Reactivity||Human, Mouse, Rat|
|Calculated MW||55647 Da|
|Homology||Mouse, rat, human - 12/13 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily F member 1, Voltage-gated potassium channel subunit Kv51, Kcnf1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)K(S)VWKFLEKPESS, corresponding to amino acid residues 166- 178 of mouse KV5.1 (Accession Q7TSH7). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated K+ channels are involved in maintaining membrane potential and regulating cell volume. They are also important for determining the cellular response to the external stimulus of neurotransmitters and neuro-peptides1. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Voltage-gated K+ channels are transmembrane proteins consisting of four α-subunits arranged in radially symmetric fashion around a central aqueous pore. Each α-subunit consists of six transmembrane segments (S1–S6) with cytoplasmic NH2- and COOH-termini 2. In mammals, a large family of genes encodes α-subunits: KV1 (homologous to Drosophila Shaker), KV2 (Shab), KV3 (Shaw), KV4 (Shal), KV5, KV6, and KV8 3. New electrically silent α subunits have recently been cloned which constitute three new subfamilies: KV5.1, KV6.1, and KV8.1. These α subunits, although cannot generate K+ channel activity by themselves, can modulate in a specific way the function of KV2.1 and KV2.2 subunits. They inhibit the KV2.1 and KV2.2 channels when expressed at high concentrations4. KV5.1 is expressed in heart, skeletal muscle, brain, kidney, and pancreas. A recent study revealed the expression of KV5.1 in isolated urinary bladder smooth muscle (UBSM) myocytes5.
References 1. Su, K. et al. (1997) Biochem. Biophys. Res. Commun. 241, 675. 2. Bocksteins, E. and Snyders, D.J. (2012) Physiology (Bethesda) 27, 23. 3. Nelson, M.T. and Quayle, J.M. (1995) Am. J. Physiol. 268, C799. 4. Kramer, J.W. et al. (1998) Am. J. Physiol. 274, C1501. 5. Salinas, M. et al. (1997) J. Biol. Chem. 272, 24371. 6. Thorneloe, K.S. and Nelson, M.T. (2003) J. Physiol. 549, 65.
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