|Reactivity||Human, Mouse, Rat|
|Calculated MW||55866 Da|
|Homology||Mouse, human - identical.|
|Other Names||Potassium voltage-gated channel subfamily S member 3, Delayed-rectifier K(+) channel alpha subunit 3, Voltage-gated potassium channel subunit Kv93, Kcns3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EFQNEDGEVDDPVLE, corresponding to amino acid residues 209-223 of rat Kv9.3 (Accession O88759). 1st extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
K+ channels are transmembrane proteins expressed in many excitable and non-excitable cells. Functional entities are formed by the tetrameric assembly of α subunits which could be done in a homomeric or heteromeric fashion. In addition, the association of β subunits is also required for the proper function of K+ channels. Various splice variants are also expressed, thereby complicating the picture1,2. K+ channels belonging to the K V9 subfamily resemble to the delayed-rectifier class of K+ channel α subunits. These channels include six transmembrane domains, an ion selective pore, a leucine zipper and positively charged amino acids in S4 the voltage sensor domain. Interestingly, both KV9.1 and KV9.3 channels are electrically silent delayed rectifying K+ channels. However, they are responsible for modifying the activity of other K+ channels such as that of KV2.1, yielding currents different from those of KV2.1 on its own1. KV9.1 is mainly expressed in the brain, human lens epithelial cells, kidney, prostate and testis. That of KV9.3 is more generalized and ubiquitous1. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat KV9.3. Anti-KV9.3 (extracellular) antibody (#AG1084) can be used in western blot analysis. It has been designed to recognize KV9.3 from human mouse and rat samples.
References 1. Shepard, A.R. and Rae, J.L. (1999) Am. J. Physiol. 277, 412. 2. Richardson, F.C. and Kaczmarek, L.K. (2000) Hear. Res. 147, 21. 3. Costigan, M. et al. (2010) Brain 133, 2519.
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