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>   home   >   Products   >   Primary Antibodies   >   KV9.1 (extracellular) Antibody   

KV9.1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - KV9.1 (extracellular) Antibody AG1085-050
    Western blot analysis of mouse (lanes 1 and 4), and rat (lanes 2 and 5) brain membranes and human HL-60 promyelocytic leukemia cell lysates (lanes 3 and 6):
    1-3. Anti-Kv9.1 (extracellular) antibody (#AG1085), (1:200).
    4-6. Anti-Kv9.1 (extracellular) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O88758
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 54915 Da
Homology Mouse, human - 12/13 amino acid residues identical.
Additional Information
Gene ID 117023
Other Names Potassium voltage-gated channel subfamily S member 1, Delayed-rectifier K(+) channel alpha subunit 1, Voltage-gated potassium channel subunit Kv91, Kcns1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EDVRDDPVLRRLE, corresponding to amino acid residues 233-245 of rat KV9.1 (Accession O88758). 1st extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Citations (0)

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K+ channels are transmembrane proteins expressed in many excitable and non-excitable cells. Functional entities are formed by the tetrameric assembly of a subunits which could be done in a homomeric or heteromeric fashion. In addition, the association of b subunits is also required for the proper function of K+ channels. Various splice variants are also expressed, thereby complicating the picture1,2. K+ channels belonging to the K V9 subfamily resemble to the delayed-rectifier class of K+ channel a subunits. These channels include six transmembrane domains, an ion selective pore, a leucine zipper and positively charged amino acids in S4 the voltage sensor domain. Interestingly, both KV9.1 and KV9.3 channels are electrically silent delayed rectifying K+ channels. However, they are responsible for modifying the activity of other K+ channels such as that of KV2.1, yielding currents different from those of KV2.1 on its own1. KV9.1 is mainly expressed in the brain, human lens epithelial cells, kidney, prostate and testis. That of KV9.3 is more generalized and ubiquitous1. Interestingly, a polymorphism in the gene encoding for KV9.1 was identified and associated with high risks of suffering from neuropathic pain3. Evidently, additional work needs to be done to evaluate and decipher the role of KV9.1 (if at all) in chronic pain states. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat KV9.1. Anti-KV9.1 (extracellular) antibody (#AG1085) can be used for western blot analysis. It has been designed to recognize KV9.1 from human mouse and rat samples.


References 1. Shepard, A.R. and Rae, J.L. (1999) Am. J. Physiol. 277, 412. 2. Richardson, F.C. and Kaczmarek, L.K. (2000) Hear. Res. 147, 21. 3. Costigan, M. et al. (2010) Brain 133, 2519.

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$ 367.00
$ 475.00
Cat# AG1085-050
Availability: 2-3 weeks
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