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>   home   >   Products   >   Primary Antibodies   >   KCNE4 (MiRP3) Antibody   

KCNE4 (MiRP3) Antibody

Affinity purified polyclonal antibody

  • WB - KCNE4 (MiRP3) Antibody AG1086-025
    Western blot analysis of rat pancreas membranes (lanes 1 and 3) and rat heart lysate (lanes 2 and 4):
    1-2. Anti-KCNE4 (MiRP3) antibody (#AG1086), (1:600).
    3-4. Anti-KCNE4 (MiRP3) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9WTW3
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 18639 Da
Homology Rat, human-identical.
Additional Information
Gene ID 57814
Other Names Potassium voltage-gated channel subfamily E member 4, MinK-related peptide 3, Minimum potassium ion channel-related peptide 3, Potassium channel subunit beta MiRP3, Kcne4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GYMKSKRREKKSS, corresponding to amino acid residues 56- 68 of mouse KCNE4 (Accession Q9WTW3). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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The K+ voltage-gated channel subfamily E member (KCNE) family is a group of small, non-conducting, single transmembrane domain proteins that associate with pore-forming potassium channel subunits to form mixed complexes with unique characteristics1.   Five different KCNE proteins have been described (KCNE1-5)2. The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the α subunits1. MinK-related peptides (MiRPs) MiRP3, protein encoded by KCNE43. The importance of these proteins to normal physiology of the heart and nervous system is exposed by their association with clinical disorders such as congenital long QT syndrome, drug-induced cardiac arrhythmias, sensorineural deafness, and periodic paralysis4. In addition, MiRPs influence the normal physiology of endocrine and exocrine glands, intestinal secretion, and renal excretion. MiRP3 is found to co-localize with KV4.2 subunits that contribute to cardiac transient outward K+ currents5. Levels of the KCNE4 transcript in human cardiac ventricle are robust and increase in patients with congestive failure suggesting a regulatory function for MiRP3 in the heart6. In addition to regulating KV4.2, MiRP3 also regulates KCNQ1 and BK channels2,5.


References 1. Abbott, G.W. et al. (1999) Cell 97, 175. 2. Grunnet, M. et al. (2002) J. Physiol. 542,119. 3. McCrossan, Z.A. and Abbott, G.W. (2004) Neuropharmacology 47, 787. 4. Levy, D.I. et al. (2008) Am. J. Physiol. 295, F380. 5. Levy, D.I. et al. (2010) J Physiol. 588, 2657. 6. Bendahhou, S. et al. (2005) Cardiovasc. Res. 67, 529.

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Cat# AG1086-025
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