|Reactivity||Human, Mouse, Rat|
|Calculated MW||44710 Da|
|Homology||Mouse, human - identical.|
|Other Names||Voltage-gated potassium channel subunit beta-1, K(+) channel subunit beta-1, Kv-beta-1, Kcnab1, Kvb1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KV־²1 (Accession P63144). N-terminus.This antibody will recognize the "canonical" splice isoform of human Kv־²1 (also known as KV־²1.3). It won't recognize splice variants KV־²1.1 and KV־²1.2. For more information see human KV־²1 entry Q14722.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1. The duration of the open states of K+ channels is determined by the rates of deactivation and inactivation. KV channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits2. The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of KVβ1 and KVα family channels3. There are three mammalian KVβ genes: KVβ1, KVβ2 and KVβ3. Additional variability in the KVβ1 family results from alternative splicing in the amino terminal region, thus yielding the KVβ1.1, KVβ1.2, and KVβ1.3 subunits4.
References 1. Hille, B. (1992) Sinauer, Sunderland, MA. 2. Zagotta, W.N. et al. (1990) Science 250, 56. 3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344. 4. McCormack, K. et al. (1995) FEBS Lett. 370, 32.
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