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KVBeta1 Antibody

Affinity purified polyclonal antibody

  • WB - KVBeta1 Antibody AG1087-050
    Western blot analysis of human SH-SY5Y neuroblastoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6): 1-3. Anti- KVβ1 antibody (#AG1087), (1:200).
    4-6. Anti- KVβ1 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P63144
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44710 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 29737
Other Names Voltage-gated potassium channel subunit beta-1, K(+) channel subunit beta-1, Kv-beta-1, Kcnab1, Kvb1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KV־²1 (Accession P63144). N-terminus.This antibody will recognize the "canonical" splice isoform of human Kv־²1 (also known as KV־²1.3). It won't recognize splice variants KV־²1.1 and KV־²1.2. For more information see human KV־²1 entry Q14722.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1. The duration of the open states of K+ channels is determined by the rates of deactivation and inactivation. KV channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits2.    The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of KVβ1 and KVα family channels3. There are three mammalian KVβ genes: KVβ1, KVβ2 and KVβ3. Additional variability in the KVβ1 family results from alternative splicing in the amino terminal region, thus yielding the KVβ1.1, KVβ1.2, and KVβ1.3 subunits4.


References 1. Hille, B. (1992) Sinauer, Sunderland, MA. 2. Zagotta, W.N. et al. (1990) Science 250, 56. 3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344. 4. McCormack, K. et al. (1995) FEBS Lett. 370, 32.

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$ 475.00
$ 575.00
Cat# AG1087-050
(40 western blots)
Availability: 2-3 weeks
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