|Calculated MW||139615 Da|
|Homology||Mouse- 12/13 amino acid residues identical.|
|Other Names||Potassium channel subfamily T member 1, Sequence like a calcium-activated potassium channel subunit, Kcnt1, Slack|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KQEEKQNRRGLAG, corresponding to amino acid residues 619-631 of rat Slack (Accession Q9Z258). Intracellular, C-terminal.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
KCa4.1 (Na+ activated K+ channel, Slack, Slo2.2, KCNT1) is a member of a subgroup within the Ca2+ activated K+ channel family. The Slack (sequence like a Ca2+-activated K+ channel) and Slick (sequence like an intermediate conductance K+ channel, KCNT2) genes, which encode Na+-activated K+ (KNa) channels, are expressed at high levels in neurons in several areas of the nervous system1. Although KCa4.1 is functionally a Na+ activated K+ channel, it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels. The family’s protein topology consists of six transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for KCa4.1 channels is composed of four subunits that can assemble as either homo or heteromers with KCNT2 (Slick). KCa4.1 and Slick subunits coassemble to form heteromeric channels that differ from the homomers in their unitary conductance, kinetic behavior, subcellular localization, and response to activation of protein kinase C. Heteromer formation requires the N-terminal domain of Slack-B, one of the alternative splice variants of the KCa4.1 channel. This cytoplasmic N-terminal domain of Slack-B also facilitates the localization of heteromeric KNa channels to the plasma membrane2. K+ channels sensitive to intracellular Na+, termed KNa channels, shape neuronal excitability. KNa channels contribute to the adaptation of firing rates and to slow after-hyperpolarizations that follow repetitive firing. In certain neurons, they also appear to be activated by Na+ influx accompanying single action potentials. Their molecular properties also suggest that these channels contribute to the response of neurons to hypoxia1. KCa4.1 channels were shown to be expressed also in dorsal root ganglion (DRG) neurons, where these channels were found to contain a NAD+ binding sequence and to be modulated by this endogenous compound3. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat KCa4.1 channel. Anti-KCa4.1 (Slack) antibody (#AG1089) can be used in western blot applications, and was designed to recognize KCa4.1 from rat and mouse samples.
References 1. Bhattacharjeea, A and Kaczmarek, L. K. (2005) TiNS 28, 422. 2. Chen, H. et al. (2009) J. Neurosci. 29, 5654. 3. Tamsett, T. J. et al. (2009) J. Neurosci. 29, 5127.
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