|Calculated MW||47936 Da|
|Homology||Mouse - identical; human 14/15 amino acid residues-identical.|
|Other Names||Inward rectifier potassium channel 16, BIR9, Inward rectifier K(+) channel Kir51, Potassium channel, inwardly rectifying subfamily J member 16, Kcnj16|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat Kir5.1 (Accession P52191).ֲ ֲ Intracellular, C-terminal.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kir5.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assembly as either homo or heteromers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.1 Kir5.1 can co-assemble with other Kir channels such as Kir2.1, Kir4.1 and Kir4.2. In fact, heteromeric Kir4.1/ Kir5.1 form the basolateral small-conductance K+ channel in distal nephron segments that is responsible for generating the basolateral membrane potential that determines the magnitude and direction of K+ diffusion from cell to peritubular fluid in the nephron.2 The Kir5.1 homomeric channel was largely thought to be non-functional, although recent evidence demonstrates that Kir5.1 homomers can form functional channels when co-expressed with the anchor protein PSD-95 in the brain where it is abundantly expressed.3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of the rat Kir5.1 channel. Anti-Kir5.1 antibody (#AG1090) can be used in Western blot and immunohistochemical applications, and was designed to recognize Kir5.1 from rat, mouse and human samples.
References 1. Bond, C.T. et al. (1994) Rec. Channels 2, 183. 2. Hebert, S.H. et al. (2005) Physiol. Rev. 85, 319. 3. Tanemoto, M. et al. (2002) Neuron 34, 387.
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