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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   K2P18.1 (TRESK) (extracellular) Antibody   

K2P18.1 (TRESK) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - K2P18.1 (TRESK) (extracellular)  Antibody AG1091-025
    Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes:
    1, 2. Anti-K2P18.1 (TRESK) (extracellular) antibody (#AG1091), (1:200).
    3, 4. Anti-K2P18.1 (TRESK) (extracellular), preincubated with the control peptide antigen.
  • WB - K2P18.1 (TRESK) (extracellular)  Antibody AG1091-025
    Western blot analysis of mouse brain membranes:

    1. Anti-K2P18.1 (TRESK) (extracellular) antibody (#AG1091), (1:200).
    2. Anti-K2P18.1 (TRESK) (extracellular) antibody, preincubated with the control peptide antigen.

  • IHC - K2P18.1 (TRESK) (extracellular)  Antibody AG1091-025
    Expression of K2P18.1 in rat dorsal root ganglia Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti- K2P18.1 (TRESK) (extracellular) antibody (#AG1091), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining).
  • ICC - K2P18.1 (TRESK) (extracellular)  Antibody AG1091-025
    Expression of K2P18.1 in ND7/23 cell line Immunocytochemical staining of K2P18.1 channel in a mouse/rat neuroblastoma x dorsal root ganglion neuron hybrid cell line (ND7/23). A. Cells were stained with Anti- K2P18.1 (TRESK) (extracellular) antibody (#AG1091), (1:50) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Show visible light images of the cells shown on (A).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6VV64
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 44403 Da
Homology Rat- 13/14 residues identical.
Additional Information
Gene ID 332396
Other Names Potassium channel subfamily K member 18, Two-pore-domain potassium channel TRESK, Kcnk18, Tresk-2, Tresk2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse K2P18.1 (Accession Q6VV64 ).ֲ 1st extracellular loop.Unlikely to recognize human samples.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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K2P18.1 (also named TWIK-related spinal cord K+ channel, TRESK or KCNK18) is a member of the 2-pore (2P) domain K+ channels family that in mammals includes 15 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1   The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer.   Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.   K2P18.1 is the only K2P channel so far, whose current is activated following Gaq-receptor coupled activation. The enhancement of K2P18.1 current involves activation of calcineurin (calcium–calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gaq activation.2  In addition, K2P18.1 is potently activated by clinical concentrations of volatile anesthetics.3   K2P18.1 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.   K2P18.1 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.   Abgent is pleased to offer a highly specific Anti-K2P18.1 (TRESK) (extracellular) antibody (#AG1091) directed against a well conserved epitope located in the first extracellular region of the mouse K2P18.1 channel. Anti-K2P18.1 (TRESK) (extracellular) antibody can be used in western blot, immunohistochemical, and immunocytochemical applications and will recognize K2P18.1 from rat and mouse samples.


References 1. Goldstein, S.A.N. et al.  (2003) Nat. Rev. Neurosci. 2, 175. 2. Mathie, A. (2007) J. Physiol. 578, 377. 3. Liu, C. et al.  (2004)  Anesth. Analg. 99, 1715.

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Cat# AG1091-025
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