|Calculated MW||44403 Da|
|Homology||Rat- 13/14 residues identical.|
|Other Names||Potassium channel subfamily K member 18, Two-pore-domain potassium channel TRESK, Kcnk18, Tresk-2, Tresk2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse K2P18.1 (Accession Q6VV64 ).ֲ 1st extracellular loop.Unlikely to recognize human samples.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P18.1 (also named TWIK-related spinal cord K+ channel, TRESK or KCNK18) is a member of the 2-pore (2P) domain K+ channels family that in mammals includes 15 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1 The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer. Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc. K2P18.1 is the only K2P channel so far, whose current is activated following Gaq-receptor coupled activation. The enhancement of K2P18.1 current involves activation of calcineurin (calcium–calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gaq activation.2 In addition, K2P18.1 is potently activated by clinical concentrations of volatile anesthetics.3 K2P18.1 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen. K2P18.1 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia. Abgent is pleased to offer a highly specific Anti-K2P18.1 (TRESK) (extracellular) antibody (#AG1091) directed against a well conserved epitope located in the first extracellular region of the mouse K2P18.1 channel. Anti-K2P18.1 (TRESK) (extracellular) antibody can be used in western blot, immunohistochemical, and immunocytochemical applications and will recognize K2P18.1 from rat and mouse samples.
References 1. Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175. 2. Mathie, A. (2007) J. Physiol. 578, 377. 3. Liu, C. et al. (2004) Anesth. Analg. 99, 1715.
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