|Calculated MW||45081 Da|
|Homology||Mouse - identical; human - 15/16 amino acid residues identical.|
|Other Names||Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited potassium channel 1, THIK-1, Kcnk13|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat K2P13.1 (Accession Q9ERS0).ֲ 1stֲ extracellularֲ loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P13.1 (also named Tandem Pore Domain Halotane Inhibited K+ channel, THIK -1or KCNK13) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 15 members. These channels show little time or voltage dependence and are considered to be “leak” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.1 The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. It has been proposed that the functional channel unit is a dimer. K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to classical K+ channel blockers. The K2P13.1 channel is activated by arachidonic acid and inhibited by the volatile anesthetic halothane. The channel is relatively widely expressed in peripheral tissues such as kidney, heart, lung and liver but it is also expressed in brain. The physiological function of the K2P13.1 channel is not clear but an involvement in the physiological response to reduced oxygen levels (hypoxia) has been proposed.2 Abgent is pleased to offer a highly specific antibody directed against an epitope in the extracellular 1st loop of the rat K2P13.1 channel. Anti- K2P13.1 (THIK-1) (extracellular) antibody (#AG1092) can be used in Western blot and immunohistochemical applications, and recognizes K2P13.1 channel from mouse and rat samples.
References 1. Rajan, S. et al. (2001) J. Biol. Chem. 276, 7302. 2. Campanucci, V.A. et al. (2005) Neuroscience, 135, 1087.
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