|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||102096 Da|
|Homology||Mouse - identical; human - 13/15 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily B member 2, CDRK, Voltage-gated potassium channel subunit Kv22, Kcnb2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CRQDIYQAVGEVKKD, corresponding to amino acid residues 859-873 of rat Kv2.2 (Accession Q63099).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV2.2 is a member of the voltage-gated K+ channel superfamily. Together with the closely related KV2.1 protein they form the KV2 subfamily also known as Shab. As with all KV channels, KV2.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homotetramer or a heteromer of KV2.2 and KV2.1 subunits. Both KV2.2 and KV2.1 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits KV8 and KV9 can modulate biochemical and biophysical properties of KV2.2. KV2.2 is expressed in the brain and in peripheral tissues such as gastrointestinal and vascular smooth muscle. Physiologically, KV2.2 has a role in maintaining membrane potential and in modulating electrical excitability in neurons. In smooth muscle cells, KV2.2 is probably involved in maintaining the contractile capacity of the cells. The pharmacology of the KV2.2 channel is somewhat limited. The channel is blocked by the classical K+ channel blockers tetraethylammonium (TEA) and 4-aminopyridine (4-AP). More recently, the small peptide toxin Stromatoxin-1 (#STS-350) has been found to be a potent and more specific inhibitor of the KV2 subfamily.
References 1. Hwang, P.M. et al. (1992) Neuron 8, 473. 2. Schmalz, F. et al. (1998) Am. J. Physiol. 274, G901. 3. Blaine, J.T. and Ribera, A.B. (2001) J. Neurosci. 21, 1473. 4. Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48.
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