|Reactivity||Human, Mouse, Rat|
|Calculated MW||41021 Da|
|Homology||Mouse, human, rabbit - identical.|
|Other Names||Voltage-gated potassium channel subunit beta-2, K(+) channel subunit beta-2, Kv-beta-2, Kcnab2, Ckbeta2, Kcnb3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat Kv־²2 (Accession P62483).ֲ ֲ N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kvβ2 (or KCNAB2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are Kvβ1 and Kvβ3. The Kvβ subunits were originally identified using a biochemical approach that demonstrated that the ion-conducting a subunits existed in a macromolecular complex with auxiliary β subunits with a probable stoichiometry of α4β4. It is now widely established that the regulatory β subunits are able to alter both the biophysical (i.e. acceleration of inactivation kinetics) and biochemical (promote cell surface expression) properties of the functional Kv channel. The Kvβ regulatory subunits are cytosolic proteins with conserved C-termini and variable N-terminus domains. The interaction with the a subunits is via the conserved C-terminal end that binds to a specific sequence in the N-terminus domain of the a subunits. Kvβ2, as well as the other Kvβ subunits can bind to all the members of the large Kv1.x (Shaker) channel subfamily. However, recent evidence suggests that it can also bind to members of the Kv4.x subfamily. Kvβ2 is the most abundant β subunit in the brain where it couples with all the Kv1.x subunits, but the protein is also expressed in peripheral tissues including heart, lung and cells of hematopoietic origin such as T cells where it couples with Kv1.3, the dominant voltage-dependent K+ channel in these cells. We have now developed a highly specific antibody directed against a unique region in the N-terminus end of the rat Kvβ2 protein. The antibody will also recognize Kvβ2 samples from mouse and human origin.
References 1. Parcej, D.N. et al. (1992) Biochemistry 31, 11084. 2. Rettig, J. et al. (1994) Nature 369, 289. 3. Pongs, O. et al. (1999) Ann. N. Y. Acad. Sci. 868, 344. 4. Li, Y. et al. (2006) Neuroscientist 12, 199.
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