|Calculated MW||111404 Da|
|Homology||Human - identical.|
|Other Names||Potassium voltage-gated channel subfamily H member 4, Brain-specific eag-like channel 2, BEC2, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Voltage-gated potassium channel subunit Kv123, Kcnh4, Elk1, Elk3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CRLNQEISRLNQEVS, corresponding to amino acids 881-895 of rat Kv12.3 (Accession Q9R1T9). Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv12.3 (also known as elk3 and KCNH4) is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (Kv10) with two members, the eag-related channels (erg or Kv11) and the eag-like K+ channels (elk or Kv12) with three members each. As all members of the family, Kv12.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with all voltage-dependent K+ channels the functional channel is a tetramer composed of four subunits. It has been suggested that the Kv12 subfamily members can form functional heteromultimers within the subfamily.1,2 Kv12.3 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as testis and lung.3 The physiological function of the Kv12.3 channel hasn’t been established, although a role in the modulation of overall excitability of neurons has been suggested.3 At the moment there isn’t much pharmacological data concerning the modulation of the Kv12.3 channel except for blocking of the channel by Ba2+.3 Abgent is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal part of the rat Kv12.3. Anti-Kv12.3 (Elk3) antibody (#AG1097) can be used in Western blot and immunohistochemical applications, and recognizes Kv12.3 from rat and human samples.
References 1. Engeland, B. et al. (1998) J Physiol 513, 647. 2. Miyake, A. et al. (1999) J Biol Chem 274, 25018. 3. Gutman, G.A. et al. (2005) Pharmacol. Rev. 57, 473.
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