|Reactivity||Human, Mouse, Rat|
|Calculated MW||109925 Da|
|Homology||Rat, chicken - identical; mouse - 13/14 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related gene potassium channel 2, ERG-2, Eag-related protein 2, Ether-a-go-go-related protein 2, hERG-2, hERG2, Voltage-gated potassium channel subunit Kv112, KCNH6, ERG2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human Kv11.2 (Accessionֲ Q9H252).ֲ ֲ Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv11.2 (erg2) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins Kv11.1 (erg1) and Kv11.3 (erg3) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the Kv11 subfamily members can form functional heteromultimers within the subfamily. Kv11.2 produces currents characterized by strong inward rectification with slow activation and very rapid inactivation kinetics, which closely resemble those produced by the much studied channel Kv11.1. The expression of Kv11.2 seems to be limited to the brain and the pituitary gland. The same is true for the Kv11.3 protein, while Kv11.1 is more widely expressed. From a pharmacological point of view, Kv11.2 channels can be blocked by well characterized organic blockers such as the anti-arrhythmic drug E-4031 (#E-500). In addition, the peptide toxins Ergtoxin-1 (#RTE-450) and BeKm-1 (#RTB-470), block Kv11.2 with different potencies. Alomone has now produced a new Anti-Kv11.2 antibody directed against a highly conserved epitope of the human protein. The antibody was used to detect Kv11.2 in rat samples using both Western blotting and immunohistochemistry.
References 1. Shi, W.M. et al. (1997) J. Neurosci. 17, 9423. 2. Wimmers, S. et al. (2001) Pflugers Arch. 441, 450. 3. Schwarz, J.R. and Bauer, C.K. (2004) J. Cell. Mol. Med. 8, 22.
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