|Calculated MW||123231 Da|
|Homology||Mouse -14/15 amino acid residues identical; human -13/15 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily H member 8, Ether-a-go-go-like potassium channel 3, ELK channel 3, Voltage-gated potassium channel subunit Kv121, Kcnh8, Elk1, Elk3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide EDKKEDRAKGRSRAG(C), corresponding to amino acid residues 143-157 of rat Kv12.1 (Accession Q9QWS8).ֲ ֲ Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv12.1 is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. They are the eag (Kv10) with two members, the eag-related channels (erg or Kv11) and the eag-like K+ channels (elk or Kv12) with three members each. As all members of the family, Kv12.1 possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with all voltage-dependent K+ channels the functional channel is a tetramer composed of four subunits. It has been suggested that the Kv12 subfamily members can form functional heteromultimers within the subfamily. Kv12.1 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as testis, colon and lung. The physiological function of the Kv12.1 channel hasn’t been established, although a role in the modulation of overall excitability of neurons has been suggested. At the moment there isn’t much pharmacological data concerning the modulation of the Kv12.1 channel except for blocking of the channel by Ba2+. We have now produced a new Anti-Kv12.1 antibody that is directed against a highly conserved epitope of the rat protein. The antibody will probably recognize also human and mouse samples.
References 1. Shi, W. et al. (1998) J. Physiol. 511, 675. 2. Zou, A. et al. (2003) Am. J. Physiol. Cell. Physiol. 285, C1356.
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