|Reactivity||Human, Mouse, Rat|
|Calculated MW||134901 Da|
|Homology||Human - identical; mouse - 15/16 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related gene potassium channel 3, ERG-3, Eag-related protein 3, Ether-a-go-go-related protein 3, Voltage-gated potassium channel subunit Kv113, Kcnh7, Erg3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat Kv11.3 (Accession O54852).ֲ ֲ Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Kv11.3 (erg3) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins Kv11.1 (erg1) and Kv11.2 (erg2) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with all voltage-dependent K+ channels the functional channel is a tetramer composed of four subunits. It has been suggested that the Kv11 subfamily members can form functional heteromultimers within the subfamily. The current of the Kv11.1 and Kv11.2 channels is that of a strong inward rectifier with slow activating kinetics. The Kv11.3 channel has different biophysical properties: it is a weak inward rectifier that is activated at negative potentials and has rapid activating kinetics. Kv11.3 expression is believed to be concentrated mainly in the brain with the stronger expression detected in the cerebral cortex and hippocampus. At the moment there is not much pharmacological information concerning Kv11.3 modulation, but the channel has been shown to be inhibited (as are all Kv11 subfamily members) by the anti-arrhythmic E-4031 as well as by the anti-psychotic drugs sertindole and pimozide. We have now produced a new Anti-Kv11.3 antibody that is directed against a highly conserved epitope of the rat protein. The antibody will also react with human and mouse samples.
References 1. Shi. W.M. et al. (1997) J. Neurosci. 17, 9423. 2. Wimmers. S. et al. (2001) Pflugers Arch. 441, 450. 3. Papa, M. et al. (2003) J. Comp. Neurol. 466, 119.
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