|Calculated MW||42704 Da|
|Homology||Rat, mouse, dog - identical.|
|Other Names||Potassium channel subfamily K member 4, TWIK-related arachidonic acid-stimulated potassium channel protein, TRAAK, Two pore potassium channel KT41, Two pore K(+) channel KT41, KCNK4, TRAAK|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human K2P4.1 (Accession Q9NYG8). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
K2P4.1 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or KCNK4) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 15 members. These channels show little time or voltage dependence and are considered to be “leaky” or “background” K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P4.1 can be integrated to a K2P subfamily that includes K2P2.1 (TREK1) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid, high intracellular pH and mechanical stretch. K2P4.1 expression is largely confined to the brain in mouse samples while in humans it is also expressed in placenta and to a lesser degree in kidney, small intestine and prostate. We have now produced a novel antibody directed against a highly conserved epitope in the intraracellular C-terminal region of the human K2P4.1 channel. The antibody will also react with rat and mouse samples.
References 1. Lesage, F. et al. (2000) FEBS Lett. 471, 137. 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793. 3. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648.
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