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>   home   >   Products   >   Primary Antibodies   >   Kir6.1 Antibody   

Kir6.1 Antibody

Affinity purified polyclonal antibody

  • WB - Kir6.1 Antibody AG1104-025
    Western blot analysis of rat heart membranes:
    1. Anti-Kir6.1 antibody (#AG1104), (1:200).
    2. Anti-Kir6.1 antibody, preincubated with the control peptide antigen.
  • WB - Kir6.1 Antibody AG1104-025
    Western blot analysis of rat cortex lysate:
    1. Anti-Kir6.1 antibody (#AG1104), (1:200).
    2. Anti-Kir6.1 antibody, preincubated with the control peptide antigen.
  • IHC - Kir6.1 Antibody AG1104-025
    Expression of Kir6.1 in rat uterus Immunohistochemical stain of pregnant rat longitudinal section of the myometrium using Anti-Kir6.1 antibody (#AG1104), (1:50).  Strong and specific staining is evident in smooth muscles cells both in the myometrium (blue arrow) and muscular layers of blood vessels (green arrow). Peroxidase reaction with DAB were used for the color reaction. Hematoxilin is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q63664
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47963 Da
Homology Mouse, human, pig, rabbit- 15/16 amino acid residues identical.
Additional Information
Gene ID 25472
Other Names ATP-sensitive inward rectifier potassium channel 8, Inward rectifier K(+) channel Kir61, Potassium channel, inwardly rectifying subfamily J member 8, uKATP-1, Kcnj8
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1 (Accession number Q63664 ).ֲ ֲ Intracellular, C-terminal part.The antibody is specific for Kir6.1, it does not cross react with Kir6.2.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
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Kir 6.1 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir 6.1 like its close relative Kir 6.2 is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization hence coupling intracellular metabolism to cellular excitability. Kir 6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly. The functional ATP sensitive channel (KATP) is composed of octamers of four Kir 6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B. Kir 6.1 tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle. We are pleased to introduce an antibody against a highly specific region in the C-terminal intracellular domain of the rat Kir 6.1 subunit. Anti-Kir 6.1 antibody (#AG1104) should also recognize Kir 6.1 from human, mouse, pig and rabbit origin.


References 1. Baukrowitz, T. et al. (2000) Eur. J. Biochem. 267, 5842. 2. Aguilar-Bryan, L. et al. (1998) Physiol. Rev. 78, 227. 

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1104-025
(40 western blots)
Availability: 2-3 weeks
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