|Reactivity||Human, Mouse, Rat|
|Calculated MW||108291 Da|
|Homology||Mouse- identical; human -14/16 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, EAG1, r-eag, Voltage-gated potassium channel subunit Kv101, Kcnh1, Eag|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KV10.1 (Accession Q63472).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.6 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
KV10.1 is a member of the Ether-a-go-go family of voltage-activated K+ channels. To date, the family includes 8 members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) subfamily (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N and C termini. The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified. Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of rat Kv10.1. The Anti-Kv10.1 (EAG-1) antibody (#AG1105) can be used for western blot , immunohistochemical and immunocytochemical applications. It has been designed to recognize Kv10.1 from rat, human and mouse samples. The antibody is specific for KV10.1 and it will not crossreact with KV10.2.
References 1. Ludwig, J. et al. (1994) EMBO J. 13, 4451. 2. Bauer, C. K. et al. (2001) J. Membrane Biol. 182, 1. 3. Pardo, LA. et al. (1999) EMBO J. 18, 5540.
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