|Reactivity||Human, Mouse, Rat|
|Calculated MW||94393 Da|
|Other Names||Potassium voltage-gated channel subfamily C member 3, KSHIIID, Voltage-gated potassium channel subunit Kv33, Kcnc3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KSPITPGSRGRYSRDRAC, corresponding toֲ amino acid residues 701-718 of rat Kv3.3 (Accession number Q01956). Intracellular, C-terminus.Recognizes only splice variant KV3.3 ג€“1.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kv3.3 is a member of the Shaw type family of voltage-gated K+ channels that includes four members (Kv3.1-3.4). Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing. Kv3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium. In the brain its expression pattern largely overlaps that of the Kv3.1 channel suggesting that they may form functional heteromers. Indeed, mouse knockouts of both Kv3.1 and Kv3.3, but not either channel alone, display severe motor defects.
References 1. Rudy, B. et al. (2001) Trends Neurosci. 24, 517. 2. Matsukawa, H. et al. (2003) J Neurosci. 23, 7677.
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