|Reactivity||Human, Mouse, Rat|
|Calculated MW||63842 Da|
|Homology||Rat, mouse- 12/14 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily A member 3, HGK5, HLK3, HPCN3, Voltage-gated K(+) channel HuKIII, Voltage-gated potassium channel subunit Kv13, KCNA3, HGK5|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human Kv1.3.ֲ (accession number P22001). Extracellular loop between domains S1 and S2.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Human B-CLL cells (see Leanza, L. et al. (2013) in Product Citations). - Human Jurkat T-cells (1:300) (see Sanchez Miguel, D.S. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Human acute T-lymphoblastic lymphomas (1:300) (see Sanchez Miguel, D.S. et al. (2013) in Product Citations). - Mouse retina (1:200) (see Cangiano, L. et al. (2007) in Product Citations). Immunocytochemistry (IC): - Human B-cell lymphoma cells (Raji cells), (1:100) (see Kawano, T. et al. (2009) in Prodcut Citations). Indirect flow cytometry (IFC): - Human T cells (see Beeton, C. et al. (2006) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||5 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel Responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation. Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs. The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.
References 1. Chandy, K.G. et al. (2001) Toxicon 39, 1269. 2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
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