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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   sloBeta3 (KCNMB3) (extracellular) Antibody   

sloBeta3 (KCNMB3) (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - sloBeta3 (KCNMB3) (extracellular) Antibody AG1111-050
    Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4):
    1-2. Anti-sloβ3 (KCNMB3) (extracellular) antibody (#AG1111), (1:1000).
    3-4. Anti-sloβ3 (KCNMB3) (extracellular) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A7VL23
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26447 Da
Homology Mouse - 11/12 amino acid residues identical.
Additional Information
Gene ID 310303
Other Names Calcium-activated potassium channel subunit beta-3, Calcium-activated potassium channel subfamily M subunit beta-3, Maxi K channel subunit beta-3, Kcnmb3 {ECO:0000312|EMBL:BAF799241}
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)HYDEEAIRTNPK, corresponding to amino acid residues 134- 145 of rat Slo־²3 (Accession A7VL23). Extracellular loop.Not recommended for human samples.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Large-conductance, voltage- and Ca2+-activated K+ channels, also known as the BK channels, are widely expressed channels that couple changes in submembrane Ca2+ concentration to the regulation of electrical excitability1. BK channels are formed from four α-subunits arising from the Slowpoke (Slo) gene product2. In addition, in smooth muscle and cochlea, an accessory β-subunit can regulate BK channel gating profoundly.  At present, four β subunits have been cloned in mammals.  β Subunits alter the Ca2+ sensitivity and gating kinetics of BK channels, greatly contributing to BK channel diversity. On the other hand, they modify the BK channel pharmacological properties, changing toxin binding and acting as receptors for drugs3.   Regulatory β subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular loop and with NH2 and COOH terminals oriented toward the cytoplasm4. Each β subunit has a tissue-specific expression and modulates channel function uniquely which provides a major mechanism for diverse BK channel phenotypes in various tissues.   β3 is highly expressed in kidney, heart, and brain5.  The β3 gene (KCNMB3) associates with Slo1 α subunits and regulate BK channel function. In humans, the β3 gene contains four N-terminal alternative exons that produce four functionally distinct β3 subunits, β3a-d. Three variants, β3a-c, exhibit kinetically distinct inactivation behaviors6. A mutation in the β3 gene is linked to idiopathic epilepsy7.


References 1. Xia, X.M. et al. (1999) J. Neurosci. 19, 5255. 2. Shen K.Z.  et al. (1994)  Pflugers Arch 408, 98. 3. Ramanathan, K. et al. (1999) Science 283, 215. 4. Orio, P. et al. (2002) News Physiol. Sci. 17, 156. 5. Lee, U.S. et al. (2009) J. Physiol. 587, 1481. 6. Zeng, X. et al. (2008) J. Gen. Physiol. 132, 115. 7. Lorenz, S. et al. (2007) Am. J. Med. Genet. B. Neuropsychiatr. Genet. 144B, 10.

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$ 367.00
$ 475.00
Cat# AG1111-050
Availability: 2-3 weeks
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