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>   home   >   Products   >   Primary Antibodies   >   KCa3.1 (SK4, IKCa1) Antibody   

KCa3.1 (SK4, IKCa1) Antibody

Affinity purified polyclonal antibody

  • WB - KCa3.1 (SK4, IKCa1) Antibody AG1112-050
    Western blot analysis of HEK-293-KCa3.1 (1, 2) and K562 (3, 4) cells:  
    1, 3. Anti-KCa3.1 (SK4, IKCa1) antibody (#AG1112), (1:200).  
    2, 4. Anti-KCa3.1 (SK4, IKCa1) antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QYW1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 47767 Da
Homology Mouse, human, pig - identical.
Additional Information
Gene ID 65206
Other Names Intermediate conductance calcium-activated potassium channel protein 4, SK4, SKCa 4, SKCa4, IK1, KCa31, KCa4, Kcnn4, Ik1, Sk4, Smik
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCa3.1ֲ (accession number Q9QYW1). Intracellular, C-terminal part.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Immunoprecipitation (IP): - Human bronchial epithelial NuLi cells (1:100) (see Klein, H. et al.(2009) in Product Citations). Immunohistochemistry (IH): - Human chorionic plate arterial smooth muscle cell sections (8 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations). - Rat mesenteric artery parafin-embedded sections (see Hilgers, R.H. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Human chorionic plate arterial smooth muscle cells (10 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations). - Normal rat cholangiocytes (NRC) (1:200) (see Dutta, A.K. et al.(2009) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Citations (0)

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KCa3.1 is part of the Ca2+ activated K+ channels family that share the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is six transmembrane domains and intracellular N- and C-termini.  KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel.   The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia. 


References 1. Ghanshani, S. et al. (2000) J. Biol. Chem. 275, 37137. 2. Hoffman, J.F. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7366.  

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$ 475.00
$ 575.00
Cat# AG1112-050
(40 western blots)
Availability: 2-3 weeks
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