|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||47767 Da|
|Homology||Mouse, human, pig - identical.|
|Other Names||Intermediate conductance calcium-activated potassium channel protein 4, SK4, SKCa 4, SKCa4, IK1, KCa31, KCa4, Kcnn4, Ik1, Sk4, Smik|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCa3.1ֲ (accession number Q9QYW1). Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunoprecipitation (IP): - Human bronchial epithelial NuLi cells (1:100) (see Klein, H. et al.(2009) in Product Citations). Immunohistochemistry (IH): - Human chorionic plate arterial smooth muscle cell sections (8 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations). - Rat mesenteric artery parafin-embedded sections (see Hilgers, R.H. et al. (2010) in Product Citations). Immunocytochemistry (IC): - Human chorionic plate arterial smooth muscle cells (10 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations). - Normal rat cholangiocytes (NRC) (1:200) (see Dutta, A.K. et al.(2009) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
KCa3.1 is part of the Ca2+ activated K+ channels family that share the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.
References 1. Ghanshani, S. et al. (2000) J. Biol. Chem. 275, 37137. 2. Hoffman, J.F. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 7366.
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